Manu Samoa will take on the Wallabies in their final test before departing for the Rugby World Cup.
The fixture on September 7th will be the first test at the new Bankwest Stadium in Paramatta, in Western Sydney.
It will also be the first time the two countries have faced off since Samoa's famous 32-23 triumph over Australia in 2011.
Samoa Rugby Union CEO: Faleomavaega Vincent Fepuleai said: "On behalf of the SRU and the people of Samoa, we would like to thank Rugby Australia for this wonderful opportunity to participate in this test match."
"We are very excited about playing at the new Bankwest Stadium in Western Sydney and we encourage all of our Pacific Island families to come and support the Manu before we depart for our RWC campaign in Tokyo."
Manu Samoa coach Steve Jackson said they jumped at the opportunity after being approached by Australia few weeks ago.
"We obviously have got the Pacific Nations Cup ( against Tonga, USA and Fiji in July and August), which gives us three games, but then there was a substantial break in between that and us travelling to Japan so the idea was always to try and get a couple of games pre Rugby World Cup," he said.
"We've got a game lined up against Tonga, which is yet to be confirmed, to give us some game-time again but then this opportunity arose which is a week out from Rugby World Cup, which is going to be perfect preparations for us which is great."
"Extremely happy to be able to play Australia. It's exciting - Rugby World Cup year. Rugby World Cup is pretty exciting but I think to have that game leading into the Rugby World Cup it's going to be something special for our players especially and what better preparation to have than to play a top tier one nation in Australia."
Samoa and Australia have played five tests since their first match-up at the 1991 Rugby World Cup, with the Wallabies winning the first four encounters before the Manu's historic upset eight years ago.
Samoa begin their World Cup campaign 17 days later against Russia in Kumagaya, three days after Australia's opening match against Fiji in Sapporo.