A second farm in the North Island has tested positive for the cattle disease, Mycoplasma bovis.
It brings the total number of farms to test positive for the disease, which was first discovered in New Zealand last year, to 35.
Mycoplasma bovis can cause lameness, mastitis and abortions in cows.
Thirty-three of the infected farms have been in the South Island, making this only the second North Island farm to be impacted.
Ministry of Primary Industries director of response Geoff Gwyn said the affected farm was a mixed sheep and beef property in the Pahiatua area near Palmerston North, in Manawatū.
Since this was the second North Island detection it should not be considered a major turning point in the response, Mr Gwyn said.