Donald Trump has suggested Theresa May's Brexit agreement could threaten a US-UK trade deal.
The US president did not specify which aspect of the deal concerned him but told reporters the withdrawal agreement "sounds like a great deal for the EU".
No 10 insisted the deal was "very clear" the UK would have an independent trade policy so it can sign trade deals with countries around the world.
Mrs May fought off heavy criticism of her Brexit deal from MPs today.
Insisting the agreement "delivered for the British people" by regaining control of laws, money and borders, she said it would be put to an MPs vote on 11 December.
Hours later, Mr Trump told reporters outside the White House: "We have to take a look seriously whether or not the UK is allowed to trade.
"Because right now if you look at the deal, they may not be able to trade with us. And that wouldn't be a good thing. I don't think they meant that."
It would appear Mr Trump was suggesting the agreement could leave Britain unable to negotiate a free-trade agreement with the United States.
However, responding to Mr Trump's comments, a Downing Street spokesman said the Brexit withdrawal agreement struck on Sunday would allow the UK to sign bilateral deals with countries including the US.
"We have already been laying the groundwork for an ambitious agreement with the US through our joint working groups, which have met five times so far," the spokesman added.
EU trade rules currently prevent the UK forging what some might view as a more advantageous bilateral trade deal with Washington.
Under the deal agreed in Brussels, the UK would continue to trade with the US under EU rules until at least the end of the "transition period" in December 2020.
During this transition - designed to allow businesses and others to prepare for the moment new post-Brexit rules kick in - the UK will be able to negotiate and strike deals with the US.
However, they will be unable to come into force until 1 January 2021 and could be delayed further if the backstop is triggered.
Tory Brexiteers fear the "backstop" written into the withdrawal agreement - which aims to prevent the return of customs posts on the Irish border in the event no UK-EU post-Brexit trade deal being agreed - could result in Britain being tied to EU rules for the long term.
In the Commons on Monday, Mrs May acknowledged the backstop was an "insurance policy no-one wants to use" but insisted the UK would have the right to determine whether it came into force.
Labour leader Jeremy Corbyn said Mrs May had brought home a "botched deal" that would "leave the UK worse off".
And the DUP's Nigel Dodds said the backstop "was bad for the United Kingdom and bad for the economy" and absolute certainty was needed over its legal application.
Tory backbencher Mark Francois was among a host of MPs to urge PM to think again, claiming the agreement was "as dead as a dodo" and "would not get through" Parliament.